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locust bean 【植物;植物學】角豆。

locust tree

This experiment passing to grope for the carbon source constitutes of the culture medium and using t . reesei rut c - 30 induced the expression of # - mannanase ( # - 1 , 4 - mannan mannohydrolase ec 3 . 2 . 1 . 78 ) . in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t . reesei rut c - 30 , then proceeded the variable carbon source ( dragon spruce fiber , com rush pith fiber , wheat straw fiber , wheat straw xylan , corn rush pith xylan , dragon spruce mannan ) to single factor , double factor , three factor , four factor and five factor orthogonal experiment . 1 determined the activity of p - mannanase using locost bean gum as substract by the 3 , 5 - dinitosalicylic acid method , and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber , wheat straw xylan , dragon spruce mannan 在里氏木霉rutc - 30的基礎培養基( mandels營養液)中加入固定碳源乳糖和槐豆膠,然后將可變碳源(云杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、云杉甘露聚糖)進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養實驗,并以槐豆膠為底物用3 , 5二硝基水楊酸法測定培養液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含云杉纖維、麥桿木聚糖和云杉甘露聚糖的誘導培養基為最佳培養基,用該培養基培養的里氏木霉( t . reesei ) rutc - 30使其轉錄的-甘露聚糖酶( - 1 , 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ) mrna量能夠滿足rt - pcr的要求。